![]() The method requires a cytoplasmic marker and a plasma membrane marker, which are co-imaged with the tagged protein of interest. ![]() ![]() To this end, we developed a highly sensitive image analysis method to quantitatively measure plasma membrane association. Since plasma membrane associated RhoGEFs are particularly effective at activating RhoA, plasma membrane localization of TGAT was examined. We investigated whether the subcellular location of TGAT is critical for its RhoGEF activity. The RhoGEF TGAT is an oncoprotein with constitutive RhoGEF activity. Alternative splicing of the RhoGEF Trio produces TGAT. Rho guanine exchange factors (RhoGEFs) control cellular processes such as migration, adhesion and proliferation.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |